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Accession #  735 PSI-MS Name  Interim Name  BEMAD_ST
Description  Beta elimination of modified S or T followed by Michael addition of DTT
Alt. Description  threo-1,4-dimercaptobutane-2,3-diol
Alt. Description  Was DTT_ST
Composition  H(8) C(4) O S(2) Monoisotopic  136.001656 Average  136.2357
Specificity Definition 1
Site  S Position  Anywhere Classification  Chemical derivative
Comment  The addition of DTT adds 136.2 not 154.2 to Serine due to loss of water in reaction
Specificity Definition 2
Site  T Position  Anywhere Classification  Chemical derivative
Comment  The addition of DTT adds 136.2 not 154.2 to Threonine due to loss of water in reaction
Notes and References 
Source  PubMed PMID Reference  Proteomics. 2005 Feb;5(2):388-98. Links rnQuantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol.Vosseller K, Hansen KC, Chalkley RJ, Trinidad JC, Wells L, Hart GW, Burlingame AL.
Source  PubMed PMID Reference  Methods Enzymol. 2006;415:113-33. Links rnIdentification of O-GlcNAc sites on proteins.Whelan SA, Hart GW.
Source  PubMed PMID Reference  Mol Cell Proteomics. 2002 Oct;1(10):791-804. rnMapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modifications.Wells L, Vosseller K, Cole RN, Cronshaw JM, Matunis MJ, Hart GW.
Notes  Beta-elimination and Michael addition of dithiothreitol (DTT) to serine and threonine adds a weight of approximately 136.2.
Curator  stephenaw777 Last Modified  2017-06-15 14:01:00 Verified  No
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