Unimod, View record [
Accession #: 735
]
Accession # |
735 |
PSI-MS Name |
|
Interim Name |
BEMAD_ST |
Description |
Beta elimination of modified S or T followed by Michael addition of DTT |
Alt. Description |
threo-1,4-dimercaptobutane-2,3-diol |
Alt. Description |
Was DTT_ST |
Composition |
H(8) C(4) O S(2) |
Monoisotopic |
136.001656 |
Average |
136.2357 |
Site |
S |
Position |
Anywhere |
Classification |
Chemical derivative |
Comment |
The addition of DTT adds 136.2 not 154.2 to Serine due to loss of water in reaction |
Site |
T |
Position |
Anywhere |
Classification |
Chemical derivative |
Comment |
The addition of DTT adds 136.2 not 154.2 to Threonine due to loss of water in reaction |
Source |
PubMed PMID |
Reference |
Proteomics. 2005 Feb;5(2):388-98. Links rnQuantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol.Vosseller K, Hansen KC, Chalkley RJ, Trinidad JC, Wells L, Hart GW, Burlingame AL. |
Source |
PubMed PMID |
Reference |
Methods Enzymol. 2006;415:113-33. Links rnIdentification of O-GlcNAc sites on proteins.Whelan SA, Hart GW. |
Source |
PubMed PMID |
Reference |
Mol Cell Proteomics. 2002 Oct;1(10):791-804. rnMapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modifications.Wells L, Vosseller K, Cole RN, Cronshaw JM, Matunis MJ, Hart GW. |
Notes |
Beta-elimination and Michael addition of dithiothreitol (DTT) to serine and threonine adds a weight of approximately 136.2. |
Curator |
stephenaw777 |
Last Modified |
2017-06-15 14:01:00 |
Verified |
No |
Back to list
|