Unimod, View record [
Accession #: 736
]
| Accession # |
736 |
PSI-MS Name |
|
Interim Name |
BEMAD_C |
| Description |
Beta elimination of alkylated Cys followed by Michael addition of DTT |
| Alt. Description |
Was DTT_C |
| Composition |
H(8) C(4) O(2) S |
Monoisotopic |
120.024500 |
Average |
120.1701 |
| Site |
C |
Position |
Anywhere |
Classification |
Chemical derivative |
| Source |
PubMed PMID |
Reference |
Mol Cell Proteomics. 2002 Oct;1(10):791-804. Mapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modifications.Wells L, Vosseller K, Cole RN, Cronshaw JM, Matunis MJ, Hart GW. |
| Source |
PubMed PMID |
Reference |
Methods Enzymol. 2006;415:113-33. Links rnIdentification of O-GlcNAc sites on proteins.Whelan SA, Hart GW. |
| Source |
PubMed PMID |
Reference |
Mol Cell Proteomics. 2006 May;5(5):923-34. Epub 2006 Feb 1. Links rnO-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry.Vosseller K, Trinidad JC, Chalkley RJ, Specht CG, Thalhammer A, Lynn AJ, Snedecor JO, Guan S, Medzihradszky KF, Maltby DA, Schoepfer R, Burlingame AL. |
| Notes |
When the beta-elimination and Michael addition of dithiothreitol (DTT) (BEMAD) reaction is used with alkylated cysteine a sulfur group is lost leaving the addition of approximately 120.2 in the chemical reaction. |
| Curator |
stephenaw777 |
Last Modified |
2017-06-15 13:58:28 |
Verified |
No |
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